抑制C5L2表达对Th17细胞功能和小鼠肾移植急性排斥反应的作用

Influence of inhibiting C5L2 expression on Th17 function and kidney transplantation acute rejection reaction in mouse model

摘要:

目的：探讨补体C5a关键受体C5L2表达抑制后，对小鼠肾移植急性排斥反应模型中Th17细胞功能以及急性排斥反应的影响。方法：构建特异性C5L2干扰腺病毒，通过小鼠尾静脉注射入小鼠急性肾移植排斥模型，检测其血肌酐，ELSA检测Th17相关细胞因子白细胞介素（interleukin, IL）-6、IL-17以及肿瘤坏死因子（tumor necrosis factor，TNF）-γ的变化，同时检测小鼠肾组织病理改变。结果：成功建立小鼠急性肾移植排斥模型，将构建的C5L2干扰腺病毒尾静脉注射后，小鼠血清Cr水平下降，IL-6、IL-17以及TNF-γ水平下降，急性排斥反应减轻。结论：特异性C5L2干扰腺病毒可以抑制Th17细胞功能，减轻肾移植急性排斥反应。

Abstract:

Objective：To explore the influence of inhibiting C5L2 expression on Th17 function and kidney transplantation acute rejec－tion reaction in mouse model. Methods：The C5L2 interference adenovirus was constructed，and injected into kidney transplantation acute rejection reaction mouse model by tail vein. The change of serum creatinine（Cr） was detected. Th17 related cytokines inter－leukin（IL）-6，IL-17 and tumor necrosis factor（TNF）-γ were detected by enzyme-linked immunosorbent assay（ELSA）. Kidney pathological change was detected after 1 weeks. Results：C5L2 interference adenovirus was constructed and injected into kidney transplantation acute rejection reaction mouse model successfully. In experimental group，the levels of serum Cr decreased，IL-17，IL-6 and TNF-γ were decreased. Conclusion：C5L2 interference adenovirus can inhibit the function of Th17 cells，and can reduce the kidney transplantation acute rejection reaction in mouse model.