糖尿病肾病相关miR-130b-3p靶点的生物信息学预测

miR-130b-3p；糖尿病肾病；靶点预测；信号通路；富集分析

摘要:

目的：预测miR-130b-3p靶基因，并对靶基因的分子功能、生物学进程和信号通路进行富集分析，为深入研究糖尿病肾病相关miR-130b-3p的靶基因功能提供理论基础。方法：通过NCBI和miRbase数据库检索基因。应用Vector NTI软件进行miR-130b-3p序列分析，应用TargetScan，picTar，RNA22，PITA和miRanda软件预测靶基因，通过DAVID 和KEGG 数据库进行靶基因功能与信号通路富集分析。结果：已知成熟的不同物种miR-130b-3p核酸序列高度保守。靶基因主要富集在核质和细胞溶质，分子功能主要富集在蛋白绑定和转录因子激活。经典的miR-130b-3p靶基因集合明显富集于KEGG数据库中的TGF-β、AMPK和内吞作用等7个信号转导通路。疾病富集分析与糖尿病高度相关。结论：成功预测miR-130b-3p靶基因，部分靶基因参与的功能及信号通路与糖尿病肾病发生发展过程密切相关。

Abstract:

Objective：To predict miR-130b-3p target genes by bioinformatic analysis and enrichment analysis for its molecular func－tion，biological process and signal pathway，so as to provide a theoretical basis for the further study of miR-130b-3p associated with diabetic nephropathy. Methods：Database of target gene was retrieved from the NCBI and miRbase database. The sequences of miR-130b-3p were analyzed with Vector NTI，and miR-130b-3p target genes were predicted using TargetScan，picTar，RNA22，PITA and miRanda. Functional enrichment analysis of target genes and signal pathway enrichment analysis were carried out using DAVID and KEGG database. Results：The sequence of miR-130b-3p was highly conserved among various species. GO analysis showed that miR-130b-3p was mainly enriched in nucleoplasm and cytosol. Molecular function was mainly enriched in protein binding and transcription factor activation. Classic miR-130b-3p was enriched in seven signal transduction pathways in KEGG database，including TGF-β，AMPK and endocytosis. The disease enrichment of target genes was highly relevant to diabetic nephropathy. Conclusion：The function and signaling pathway of miR-130b-3p target genes are closely related with the development and progression of diabetic nephropathy.