新生儿与母亲孕晚期维生素A水平的相关研究

Vitamin A levels in the serum of neonates and pregnant womenin the third trimester：a correlation study

摘要:

目的：综合评价新生儿出生时维生素A（vitamin A, VA）水平，探讨其与母亲孕晚期VA水平的相关性。方法：收集2016年10至12月在重庆医科大学附属第二医院出生的正常新生儿脐静脉血及其母亲分娩前静脉血，采用高效液相色谱-串联质谱（HPLC-MS/MS）法检测血清视黄醇、视黄酯（视黄醇棕榈酰酯和视黄醇乙酸酯）及β-胡萝卜素水平，通过单因素方差分析和多元回归模型分析新生儿与孕母VA水平的相关性。结果：共收集新生儿及孕母82对，新生儿血清视黄醇、视黄醇棕榈酰酯及β-胡萝卜素浓度分别是（0.490±0.129）、（0.016±0.016）、（0.038±0.030） μmol/L，明显低于孕母（0.988±0.268）、（0.319±0.314）、（2.196±2.005） μmol/L（均P=0.000）；不同VA营养状况的孕母组间（充足组、不足组及缺乏组），新生儿脐血视黄醇、视黄醇棕榈酰酯及β-胡萝卜素水平有统计学差异（P=0.017、0.044、0.030），孕母VA充足组脐血视黄醇、视黄醇棕榈酰酯、β-胡萝卜素水平均高于VA缺乏组（均P<0.05）。其余各组间比较无统计学意义（P>0.05）。孕母血视黄醇水平为脐血视黄醇、β-胡萝卜素水平的独立影响因素（回归系数b=0.149、0. 035；95%CI=0.048~0.251、0.011~0.059）。结论：新生儿VA营养水平总体低于孕母，其视黄醇、β-胡萝卜素水平与孕母视黄醇水平具相关性。

Abstract:

Objective：To evaluate comprehensively the vitamin A（VA） status of neonates at birth and discuss the correlation with maternal VA status. Methods：From October to November 2016 in the Second Affiliated Hospital of Chongqing Medical University，blood from healthy mothers before delivery and umbilical cord blood samples from healthy neonates were collected and measured for serum retinol，retinyl esters（retinyl acetate and retinyl palmitate） and β-carotene levels by high performance liquid chromatography tandem mass spectrometry method（HPLC-MS/MS）. The single factor variance and multiple regression model were applied to analyze the correlation between neonatal umbilical and maternal serum VA levels. Results：Totally 82 mothers and their neonates were in－cluded. Retinol，retinyl palmitate and β-carotene concentration of umbilical cord blood serum were respectively（0.490±0.129），（0.016±0.016） and （0.038±0.030） μmol/L，significantly lower than those of （0.988±0.268），（0.319±0.314） and （2.196±2.005） μmol/L in maternal serum（all P=0.000）；among all groups of different maternal VA status（VA sufficient，insufficient and de－ficient groups），there were significant differences in retinol，retinyl palmitate and β-carotene level of umbilical cord blood serum（P=0.017，0.044 and 0.030，respectively）. And they were all significantly higher in VA sufficient group than in VA deficient group（all P<0.05）. There was no statistic difference between the other groups（P>0.05）. Maternal retinol level was the independent influence factor affecting both retinol and β-carotene level of umbilical cord serum（regression coefficient b=0.149，0.035，respectively；95%CI=0.048 to 0.251，0.011 to 0.059，respectively）. Conclusion：The overall neonatal VA level is lower than that of mother，and both neonatal retinol and β-carotene concentration are associated with maternal retinol concentration.