过敏性鼻炎患者血液嗜酸性粒细胞FcεRI和CD63表达研究

Expressions of FcεRI and CD63 in eosinophils of patients with allergic rhinitis

摘要:

目的：检测过敏性鼻炎（allergic rhinitis，AR）患者外周血嗜酸性粒细胞FcεRI和CD63的表达。方法：收集AR患者和健康人（healthy controls，HCs）的外周血，用蒿草花粉和尘螨过敏原粗提液刺激，流式细胞仪检测嗜酸性粒细胞富集群中FcεRI和CD63的表达情况。结果：静息状态下，AR患者[0.92%（0.70%~1.64%）]相比于HCs[0.71%（0.43%~0.97%）]嗜酸性粒细胞比例上调（Z=-2.050，P=0.041）；AR患者[6.19%（3.05%，8.19%）；1 462（1 164，1 720）]FcεRI+嗜酸性粒细胞的比例和平均荧光强度（mean fluorescence intensity，MFI）比HCs[3.16%（1.55%，4.53%）；808（524，1308）]增加（Z=-2.102，P=0.036）；AR患者[2 031 （1 725，3 338）]CD63+嗜酸性粒细胞的MFI比HCs[1 491（1 297，1 613）]升高36%（Z=-3.192，P=0.002）。0.1 μg/mL蒿草过敏原粗提液[2 618（2 131，3 315）]和1.0 μg/mL蒿草过敏原粗提液[3 108（2 317，3 792）]能诱导AR患者CD63+嗜酸性粒细胞的MFI上调（Z=-2.667，P=0.008；Z=-2.845，P=0.004）。结论：嗜酸性粒细胞表达的FcεRI和CD63参与AR发病。过敏原特异性嗜酸性粒细胞激发试验检测CD63的表达，可能成为AR诊断的补充实验。

Abstract:

Objective：To investigate the expression of FcεRI and CD63 in blood eosinophils from patients with allergic rhinitis（AR）. Methods：Peripheral blood samples were collected from AR patients and healthy controls（HCs），then incubated with crude extracts of artemisia pollen and dust mite. Expressions of FcεRI and CD63 in blood eosinophils were detected by flow cytometry. Results：With－out challenge，it was showed that the ratio of eosinophils in AR patients[0.92%（0.70% to 1.64%）] was increased（Z=-2.050，P=0.041） when compared with that of HCs[0.71%（0.43% to 0.97%）]. Percentage of FcεRI+ eosinophils and mean fluorescence intensity（MFI） of FcεRI expression on eosinophils in the blood of AR patients[6.19%（3.05% to 8.19%）；1 462（1 164 to 1 720）] were enhanced（Z=-2.102，P=0.036；Z=-2.777，P=0.007） when compared with those of HCs[3.16%（1.55% to 4.53%）；808（524 to 1 308）]，respectively. MFI of CD63+ eosinophils in AR patients[2 031（1 725 to 3 338）] was higher（Z=-3.192，P=0.002） than that of HCs[1 491（1 297 to 1 613）]. Artemisia pollen at 0.1 μg/mL[2 618（2 131 to 3 315）] and 1.0 μg/mL[3 108（2 317 to 3 792）] induced upregulation of MFI of CD63+ eosinophils in the blood of AR patients. Conclusion：FcεRI and CD63 are likely to play key roles in AR. Allergen-specific eosinophils challenge test to detect the expression of CD63 may be a supplement experiment for diagnosis of AR.