一种新型抗菌剂———噬菌体裂解酶的研究进展

Research progress of a novel antimicrobial agent-phage lytic enzyme

摘要:

随着抗生素的广泛使用，耐药菌株的不断出现给临床治疗严重感染性疾病带来严峻的挑战，新型抗菌药及制剂的研制与开发迫在眉睫。噬菌体裂解酶是由双链DNA噬菌体感染后期表达的、能裂解细菌细胞壁的一类蛋白水解酶。近年来对噬菌体裂解酶研究显示其在原核细胞中能得到成功克隆表达，且表达的裂解酶具有较强的体内外杀菌作用。大量动物实验研究表明裂解酶制剂能特异性抗宿主菌，抗菌作用迅速，对机体安全、无害，且裂解酶之间以及与传统的抗菌剂之间具有协同杀菌作用。噬菌体裂解酶的这些特性使其在临床治疗耐药感染性疾病方面具有广阔的应用前景。

Abstract:

With the widespread use of antibiotics，increasing drug-resistant strains bring more challenges to clinical serious infectious disease，therefore，the research and development of the novel antibacterial and agent is in urgency. Phage lysine is a kind of protease which is expressed in the last infectious phase of double-stranded DNA phage and has the ability to crack bacterial cell wall. In re－cent years，studies of phage lysine have showed that it can be cloned and expressed in prokaryocyte，with expressed lysins having po－tency to kill bacteria in vitro and vivo. A number of experiments on animal showed that lysin agents can specifically resist the host bacteria，featuring efficiency，specificity，safety and synergy. These characteristics of phage lysin make it possible to be applied widely in treating clinical drug-resistant diseases.