ACE基因多态性与贵州布依族、汉族原发性高血压及患者血清PⅢNT、MMP-9及 hs-CRP的相关性研究

Relationship of angiotensin converting enzymegene polymorphism，PⅢNT，MMP-9 and hs-CPR in essential hypertension in Buyi and Han populations in Guizhou province

摘要:

目的：探讨血管紧张素转化酶（angiotensin converting enzymegene，ACE）基因I/D位点多态性与贵州布依族、汉族原发性高血压及患者血清Ⅲ型前胶原氨基端肽（procollagen Ⅲ N-terminal peptide，PⅢNT）、基质金属蛋白酶-9（matrix metallopro－teinase-9，MMP-9）及高敏C反应蛋白（hypersensitive C-reactive protein，hs-CRP）的相关性。方法：入选贵州黔南地区原发性高血压患者302例（布依族154例，汉族148例）为高血压组，健康者299例（布依族143例，汉族156例）为正常对照组，应用聚合酶链反应-限制性片段长度多态性技术检测ACE I/D位点各基因型及等位基因，采用酶联免疫吸附试验检测血清PⅢNT、MMP-9及hs-CRP水平。结果：①ACE基因ID基因型及D等位基因分布频率在高血压组和正常对照组间（ ?字2=13.50，P=0.001）、在布依族高血压组和布依族正常对照组间（ ?字2=28.28，P=0.000）差异均有统计学意义。②PⅢNT、MMP-9与hs-CRP水平在布依族与汉族间、在高血压组与正常对照组间、在布依族高血压组与对照组间，以及在汉族高血压组与对照组间差异均有统计学意义（P<0.01）；PⅢNT、MMP-9与hs-CRP水平在ACE I/D位点不同基因型间差异均有统计学意义（P<0.01）。结论：①ACE基因ID基因型及D等位基因可能与布依族高血压有关；在布依族正常人群中，具有ID基因型及D等位基因者可能比具有Ⅱ、DD基因型及I等位基因者患高血压的风险高；ACE基因 I/D多态性可能是贵州黔南布依族高血压的致病因素之一。②布依族高血压患者及具有ID基因型的患者可能更易出现血管壁胶原积累、炎症反应、细胞外基质沉积及血管重塑。

Abstract:

Objective：To analyze the relationship between the polymorphism of angiotensin converting enzymegene（ACE） gene I/D，procollagen Ⅲ N-terminal peptide（PⅢNT），matrix metalloproteinase-9（MMP-9） and hypersensitive C-reactive protein（hs-CRP） of essential hypertension（EH） of Buyi and Han populations in Guizhou province. Methods：The method of polymerase chain reaction/re－striction fragment length polymorphism was used to detect the frequency of each genotype and allele from 302 EH patients（154 Buyi people，148 Han people） and 299 healthy people（143 Buyi people，156 Han people） in southeastern Qiannan Guizhou. The method of enzyme-linked immunosorbent assay was used to detect level of the PⅢNT，MMP-9 and hs-CPR of those pa－tients selected. Results：①The frequencies of ID genotype and allele D of ACE gene were statistically significant between EH group and health group，and between EH group and health control group in Buyi people（P<0.01）. ②The level of PⅢNT，MMP-9 and hs-CRP were statistically significant between Buyi people and Han people，and between EH group and health control group，and between EH group and health control group in Buyi people，and between EH group and health control group in Han people（P<0.01）. The levels of PⅢNT，MMP-9 and hs-CRP were statistically significant different in different genotypes of ACE I/D（P<0.01）. Conclusion：①The ID genotype and the allele D of ACE gene may be associated with the EH of Buyi people. Among the normal group of Buyi people，those who carry the ID genotype and allele D have higher risk than who carry Ⅱ or DD genotype and allele Ⅰ. The polymorphism of allele I/D of ACE gene may be one of the pathogenic factors of EH in Buyi people who came from southeastern Qiannan Guizhou.②The EH patients of Buyi people and those carry the ID genotype are may likely occur collagen accumulation，inflammatory reaction，deposition of extracellular and vascular remodel.