银屑病患者血液嗜酸性粒细胞中IL-18、IL-18结合蛋白和IL-18受体的表达变化及其相关性研究

Expressions and correlations of IL-18，IL-18BP and IL-18R in eosinophil-enriched blood cells from psoriasis patients

摘要:

目的：检测银屑病（psoriasis）患者血液嗜酸性粒细胞群中白介素-１８（interleukin-18，IL-18）、IL-18结合蛋白（binding protein，BP）和IL-18受体（receptor，R）的表达变化，并分析其相关性。方法：收集17例银屑病患者和18例健康人血液，用蒿草花粉、尘螨和梧桐花粉粗提液刺激，流式细胞术检测嗜酸性粒细胞富集群中IL-18、IL-18BP及IL-18R的表达，SPSS分析其相关性。结果：静息状态下，银屑病患者血液嗜酸性粒细胞富集群中IL-18+、IL-18R+ 细胞比例较正常人分别升高了3.7倍和4.1倍（Z=-3.070，P=0.002；Z=-4.753，P=0.000），而IL-18BP+ 细胞降低了87%（Z=-4.556，P=0.000）；尘螨过敏原刺激银屑病患者血液后，嗜酸性粒细胞富集群中IL-18+ 细胞比例升高约1.5倍（Z=-3.548，P=0.000）；梧桐花粉刺激银屑病患者血液后，嗜酸性粒细胞富集群IL-18BP+ 细胞升高约1.1倍（Z=-3.445，P=0.000）。此外，静息状态下银屑病患者血液嗜酸性粒细胞群中IL-18BP+细胞比例和IL-18+ 细胞比例均呈高度相关，Spearman相关系数分别为r=0.587，P=0.013。结论：上述结果提示嗜酸性粒细胞表达的IL-18、IL-18BP和IL-18R极可能参与银屑病。抗IL-18制剂和IL-18R阻断剂可能是治疗银屑病的潜在靶点。

Abstract:

Objective：To investigate the expressions of interleukin-18（IL-18），interleukin 18 binding protein（IL-18BP） and inter－leukin 18 receptor（IL-18R） in eosinophil-enriched cells from patients with psoriasis，and the correlation among them. Methods： Blood samples from psoriasis patients and healthy controls（HC） were collected and incubated with the extracts of Artemisia pollen，dust mite，and Platanus pollen. Expressions of IL-18，IL-18BP and IL-18R in blood eosinophils were detected by flowcytometry. SPSS software was employed to analyze the correlation among them. Results：Levels of IL-18+ and IL-18R+ blood eosinophils in psoriasis patients were increased up to 3.7（Z=-3.070，P=0.002） and 4.1 folds（Z=-4.753，P=0.000），respectively. While IL-18 BP+ eosinophil was reduced by 87% compared with HC when cultured with medium only（Z=-4.556，P=0.000）. Dust mite extracts induced approximately 1.5-fold increase in IL-18+ eosinophils of psoriasis patients（Z=-3.548，P=0.000），and Platanus pollen extracts induced approximately 1.1-fold elevation in IL-18BP+ expression（Z=-3.445，P=0.000）. In addition，the proportions of IL-18BP+ and IL-18+ eosinophils in psoriasis patients were highly correlated（r=0.587，P=0.013）. Conclusion：Increased expressions of IL-18，IL-18R，and decreased expression of IL-18BP in eosinophil-enriched blood cells from psoriasis patients may suggest that IL-18，IL-18R and IL-18BP expressed in eosinophils may be involved in the pathogenesis of psoriasis. Anti-IL-18 agents or IL-18R blockers might become potential targets for psoriasis treatment.