先天性巨结肠肠管lncRNA-mRNA共表达谱芯片初步筛查研究

Preliminary screening of lncRNA-mRNA coexpression microarray in Hirschsprung’s disease

摘要:

目的：研究先天性巨结肠（Hirschsprung’s disease，HD）病变段肠管与正常段肠管lncRNA-mRNA表达谱的差异。方法：采用lncRNA-mRNA共表达谱芯片筛查5例HD肠管和5例配对的正常肠管差异表达的lncRNA和mRNA，并以17对样本对其中显著差异的5个lncRNA进行qRT-PCR验证，同时应用生物信息学的方法构建lncRNA-mRNA共表达网络，预测差异lncRNA的靶基因，并对预测的靶基因进行基因本体论（gene ontology，GO）分析和通路（pathway）分析。结果：lncRNA-mRNA共表达谱芯片筛查出HD肠管中存在353个表达上调，474个下调的lncRNA，以及865个上调，461个下调的mRNA。并验证出5个差异表达的lncRNA。lncRNA与mRNA相互关联，通过调控相应的靶mRNA，参与包括细胞增殖、分化和迁移等多种生物学过程，与HD的发病有一定相关性。结论：HD肠管中存在大量差异表达的lncRNA和mRNA。lncRNA和mRNA之间具有广泛的联系，形成共表达网络，lncRNA可能是通过调控相应的靶mRNA参与HD的发病。

Abstract:

Objective：To analyze the expression profile of lncRNA-mRNA in lesion and normal intestines of Hirschsprung’s diseases （HD）. Methods：The lncRNA-mRNA microarray was used to screen the differentially expressed lncRNA and mRNA in 5 HD intestines and 5 paired normal intestines. Seventeen groups of samples were used to do the quantitative real-time polymerase chain reaction（qRT-PCR） on 5 lncRNAs with significant differences. Gene co-expression network was constructed by bioinformatics methods. Results：There were 353 up-regulated and 474 down-regulated expression of lncRNA，and 865 up-regulated and 461 down-regulated expression of mRNA. lncRNA played a role in the pathogenesis of HD，by regulating the corresponding target mRNA，participating in various biological processes like cell proliferation，differentiation，migration et al. Conclusion：There are many differentially expressed lncRNA and mRNA in HD intestines. mRNA and lncRNA are closely related，forming co-expression network. lncRNA may participate in the pathogenesis of HD through regulating the corresponding target mRNA.