Clus-human蛋白在精神分裂症患者脑脊液中的差异表达

Differential expression of Clus-human protein in cerebrospinal fluid of patients with schizophrenia

摘要:

目的：分析精神分裂症患者脑脊液中蛋白质的差异表达。方法：选取精神分裂症及非精神分裂症患者各12例，抽取脑脊液，用蛋白质分离技术———二维聚丙烯酰氨凝胶技术和蛋白质识别与鉴定技术———生物质谱技术筛选脑脊液中标志性蛋白质。结果：精神分裂症组Clus-human蛋白含量为（2.560 6±0.319 2） μg/L，对照组Clus-human蛋白含量为（2.134 9±0.418 7） μg/L，精神分裂症蛋白Clus-human含量与对照组Clus-human含量具有统计学差异（t=2.801，P=0.010）。结论：精神分裂症患者脑脊液中发现Clus-human蛋白，对精神分裂症的诊断可能具有重要意义。

Abstract:

Objective：To investigate the differential expression of proteins in the cerebrospinal fluid of patients with schizophrenia. Methods：A total of 12 patients with schizophrenia and 12 patients without schizophrenia were enrolled. Cerebrospinal fluid was collected，and two-dimensional polyacrylamide gel electrophoresis for protein separation and matrix-assisted laser desorption/ionization time-of-flight mass spectrometry for protein recognition and identification were used to screen out symbolic proteins in cerebrospinal fluid. Results：There was a significant difference in the content of Clus-human protein between the schizophrenia group and the non-schizophrenia group（2.560 6±0.319 2 μg/L vs. 2.134 9±0.418 7 μg/L，t=2.801，P=0.010）. Conclusion：Clus-human protein is found in the cerebrospinal fluid of patients with schizophrenia and may have important significance in the diagnosis of schizophrenia.