芯片技术分析卵巢子宫内膜异位症外泌体microRNA表达差异

Differential expression profiles of exosomal microRNAs in ovarian endometriosis based on the microarray technique

摘要:

目的：探讨卵巢子宫内膜异位症（ovarian endometriosis，OEM）外泌体微小RNA（microRNA，miRNA）的表达谱。方法：采用芯片技术对3例OEM患者及3例健康对照者的在位内膜间质细胞外泌体miRNA进行分析，筛选出组间差异miRNA。结果：获得存在差异表达miRNA共12条，其中hsa-miR-6829-5p、hsa-miR-5188、hsa-miR-4430、hsa-miR-584-5p、hsa-miR-4485-5p、hsa-miR-4257在实验组表达上调，hsa-miR-188-5p、hsa-miR-4741、hsa-miR-4516、hsa-miR-1229-5p、hsa-miR-7150、hsa-miR-5787表达下调。对表达差异较大的外泌体miRNA进行靶基因预测及其基因功能（gene ontology，GO）分析和信号通路（pathway）分析，发现靶基因聚集的生物学功能多数参与生物进展过程的调控。结论：OEM患者与健康对照者的在位内膜间质细胞外泌体miRNA存在着差异性表达。差异性表达的miRNA特异性很高，对进一步阐明该疾病的发病机制和寻找新的诊断标记物具有重要意义。

Abstract:

Objective：To investigate the expression profile of exosomal microRNAs（miRNAs） in ovarian endometriosis（OEM）. Methods：The microarray technique was used to analyze the exosomal miRNAs of eutopic endometrial stromal cells in three patients with OEM and 3 healthy controls，in order to screen out the differentially expressed miRNAs. Results：A total of 12 differentially expressed microRNAs were screened out；hsa-mir-6829-5p，hsa-mir-5188，hsa-mir-4430-hsa-mir-584-5p，hsa-mir-4485-5p，and hsa-mir-4257 were up-regulated in the case group，while hsa-mir-188-5p，hsa-mir-4741，hsa-mir-4516，hsa-mir-1229-5p，hsa-mir-7150，and hsa-mir-5787 were downregulated. Target gene prediction，gene ontology analysis，and pathway analysis were performed for exosomal miRNAs with significant differential expression，and the results showed that most of the biological functions of target gene aggregation were involved in the regulation of biological progression. Conclusion：There are differentially expressed exosomal miRNAs between OEM patients and healthy controls. The differentially expressed miRNAs have high specificity and can help to further clarify the pathogenesis of OEM and search for new diagnostic markers.