目的：探究锌指蛋白Snail与转录调节因子核因子－κＢ（nuclear factor－κB，NF-κB）在宫腔粘连（intrauterine adhesions，I－UAs）患者子宫内膜中的表达。方法：选择34例宫腔粘连患者作为试验组（IUAs），非宫腔粘连患者19例作为对照组（control），在宫腔镜直视下取子宫内膜组织。采用实时荧光定量PCR、免疫组织化学和Western blot分别检测 NF-κB、Snail、E-cadherin、Vimentin mRNA水平和蛋白水平的表达。结果：与对照组相比较，宫腔粘连患者子宫内膜组织中NF-κB（2.478±0.279 vs. 1.459±0.323，P=0.013）、Snail（2.801±0.502 vs. 1.218±0.313，P=0.005）、 Vimentin（3.581±0.650 vs. 1.756±0.260，P=0.006）的表达明显升高，E-cadherin（1.324±0.617 vs. 2.784±0.647，P=0.023）表达明显降低。结论：NF-κB和Snail可能参与了宫腔粘连的发生发展。
Abstract:
Objective：To examine the expression of zinc finger protein Snail and nuclear factor kappa B（NF-κB） in the endometrium of patients with intrauterine adhesions（IUAs）. Methods：Endometrial tissues were collected by hysteroscopy from 34 IUA patients（IUA group） and 19 non-IUA patients（control group）. The mRNA and protein expression levels of NF-κB，Snail，E-cadherin，and Vimentin were determined using real-time quantitative PCR，immunohistochemistry，and Western blot. Results：The IUA group had significantly increased expression of NF-κB（2.478±0.279 vs. 1.459±0.323，P=0.013），Snail（2.801±0.502 vs. 1.218±0.313，P= 0.005），and Vimentin（3.581±0.650 vs. 1.756±0.260，P=0.006），but significantly reduced expression of E-cadherin（1.324±0.617 vs. 2.784±0.647，P=0.023），as compared with the control group. Conclusion：NF-κB and Snail may be involved in the development and progression of IUA.
