卵巢浆液性癌中miR-496的表达及与SIX1的相关性

Expression of miR-496 and its correlation with SIX1 in ovarian serous carcinoma

摘要:

目的：检测卵巢浆液性癌中微小RNA-496(miR-496)的表达,关注其表达意义及与同源异型蛋白SIX1(SIX1)的相关性。方法：选择75例卵巢浆液性癌作为观察组,选择75例卵巢浆液性囊腺瘤作为对照组,应用实时荧光定量PCR法检测两组中miR-496的表达,应用免疫组化法检测观察组中PCNA和BAX的表达,应用Western Blot法检测观察组中SIX1的表达。结果：miR-496在观察组的表达量明显低于对照组(P<0.05),miR-496的表达在不同肿瘤最大径、病理分级、有无脉管累犯、是否双侧发生、有无淋巴结转移和不同TNM分期的分组中差别有统计学意义(P<0.05)。miR-496与生存时间有关(P<0.05),miR-496与PCNA(r=-0.54,P=0.018)、miR-496与SIX1(r=-0.58, P=0.013)均具有负相关性,miR-496与BAX(r=0.52,P=0.011)具有正相关性。结论：卵巢浆液性癌中miR-496的表达下降,是促进肿瘤形成和进展的重要分子因素,检测miR-496对判断预后可能有一定价值。miR-496不仅能调节细胞增殖和凋亡,还可能通过对靶基因SIX1的调控起到促进肿瘤进展的作用。

Abstract:

#$NLObjective：To detect the expression of microRNA-496(miR-496) in ovarian serous carcinoma, analyze its clinical significance, analyze its correlation with homologous heteroprotein SIX1(SIX1). Methods：75 cases of ovarian serous carcinoma were selected as the observation group, 75 cases of ovarian serous cystic adenoma were selected as the control group. Expression of miR-496 was detected by real-time fluorescence quantitative PCR in two groups. Expression of PCNA and BAX was detected by immunohistochemical method in the observation group. Expression of SIX1 was detected by Western Blot method in the observation group. Results：Expression of miR-496 was lower in the observation group than that in the control group(P<0.05). The expression of miR-496 was statistically significant in different tumor maximum diameter, histological grade, vascular recidivism, bilateral occurrence, lymph node metastasis and different TNM stages in the observation group(P<0.05). Expression of miR-496 is related to survival time(P<0.05). Negative correlation were found between miR-496 and PCNA(r=-0.54,P=0.018), miR-496 and SIX1(r=-0.58, P=0.013), positive correlation were found between miR-496 and BAX(r=0.52,P=0.011). Conclusion：Expression of miR-496 of ovarian serous carcinoma is decreased, which has a certain regulatory effect on the formation and development of the tumor. Expression of miR-496 has a certain significance for judging the prognosis. MiR-496 not only regulates cell proliferation and apoptosis, but also may play a role in the regulation of SIX1.