激光捕获显微切割技术进展与应用

Advances in laser capture microdissection and its clinical application

摘要:

激光捕获显微切割技术能从复杂的组织样品中精确地分离出纯净的单一类型细胞群或单个细胞，是研究组织或细胞特异性表达及其分子机制的常用工具，是21世纪生命科学领域不可缺少的技术。激光捕获显微切割技术现已广泛应用于缺血性脑血管疾病的研究。本文介绍了激光捕获显微切割的原理、分类、发展、应用及展望，并对激光捕获显微切割在脑缺血中的应用作了简要分析。

Abstract:

Laser capture microdissection（LCM） technique can accurately separate a single type of cells or a single cell from complex tissue samples. It is a common tool for studying the specific expression of tissue or cells and their molecular mechanism and is an indispensable technique in the field of life sciences in the 21st century. At present，LCM has been widely used in the research on is－chemic cerebrovascular diseases. This article describes the principle，classification，development，application，and perspectives of LCM and analyzes the application of LCM in cerebral ischemia.