线粒体活性对人类精子活动力的影响

Effects of mitochondrial activity on human spermatozoa motility

摘要:

目的：分析线粒体活性对人类精子活动力的影响。方法：以线粒体特异性荧光染料———mitotracker deep red 633（MT－DR）对不同活力的精子进行染色，然后用流式细胞术测定精子线粒体活性（即MTDR荧光强度值）。用雷帕霉素（rapamycin，RP）和羟氯喹（hydroxychloroquineon，HCQ）调控精子活性水平，测定精子活动力的变化。结果：MTDR荧光强度值与前向运动速率（progressive motility，PR）不相关（r=0.182，P=0.268）。5 nmol/L和100 nmol/L的RP均可明显降低线粒体活性，50 μmol/L HCQ可明显提高线粒体活性（P<0.05），但它们对精子活动力影响都不明显（P>0.05）。结论：线粒体活性对人类精子活力影响不明显。

Abstract:

Objective：To analyze effects of mitochondrial activity on human spermatozoa motility. Methods：Mitochondrial specific fluorescent dye，mitotracker deep red 633（MTDR），was used to stain sperms with different motility. And the flow cytometry was used to determine the activity of sperm mitochondria（that is，value of MTDR fluorescence intensity）. Rapamycin（RP） and hydroxychloro－quine（HCQ） were used to regulate the mitochondrial activity，and then measure the change of sperm motility. Results：MTDR fluores－cence intensity value was not correlated with spermatozoa progressive motility（PR） rate（r=0.182，P=0.268）. RP of 5 nmol/L or 100 nmol/L was able to significantly decrease the mitochondrial activity，and HCQ of 50 μmol/L was able to significantly increase the mitochondrial activity（P<0.05）. However，those above concentrations had no obvious effect on sperm motility（P>0.05）. Conclusion：There is no obvious effect of mitochondrial activity on human sperm motility.