转录因子EB对早孕小鼠子宫内膜蜕膜化的影响

Effect of transcription factor EB on endometrial decidualization in early pregnant mice

摘要:

目的：探索转录因子EB（transcription factor EB，TFEB）在围植入期小鼠子宫内膜的表达规律，初步探讨TFEB在早孕小鼠子宫内膜蜕膜化中的作用。方法：构建正常妊娠小鼠模型并分别收集早孕第4、5、6天（D4、D5、D6）的小鼠子宫内膜及D5的子宫内膜着床点组织（implantation site，IS）和着床旁组织（inter-implantation site，IIS）；构建假孕小鼠模型并分别收集假孕第4、5、6天（PD4、PD5、PD6）的小鼠子宫内膜组织；构建体内人工诱导蜕膜化模型并分别收集对照侧（induced decidualization con－trol，IDC）和诱导侧（induced decidualization，ID）的小鼠子宫内膜组织；构建体外人工诱导蜕膜化模型并分别收集对照组和诱导组的小鼠子宫内膜基质细胞。使用Western blot和Real-time PCR检测TFEB在小鼠子宫内膜中和在小鼠子宫内膜基质细胞中的mRNA和蛋白表达；使用免疫组化方法检测TFEB在小鼠D5子宫内膜中的分布和表达。结果：随着孕期天数的增加，TFEB在D4、D5、D6的表达逐渐减少；TFEB在D5子宫ＩＳ的mRNA和蛋白表达明显低于D5的子宫IIS；TFEB在蜕膜化诱导侧子宫内膜组织的mRNA和蛋白表达明显低于未诱导侧子宫内膜组织；子宫内膜基质细胞蜕膜化诱导后TFEB mRNA和蛋白表达明显低于未诱导组。结论：本研究初步揭示TFEB可能与小鼠妊娠早期子宫内膜蜕膜化有关，但其具体调节作用有待进一步深入探讨。

Abstract:

Objective：To explore the expression of transcription factor EB（TFEB） in the mouse endometrium during the peri-implan－tation period，and to preliminarily investigate the role of TFEB in endometrial decidualization in early pregnant mice. Methods：A normal pregnant mouse model was established，and the mouse endometrium on the 4th（D4），5th（D5），and 6th（D6） days of early pregnancy and the endometrial implantation site tissue（IS） and the inter-implantation site tissue（IIS） on D5 were collected；a pseu－dopregnant mouse model was established and the mouse endometrium on the 4th（PD4），5th（PD5），and 6th（PD6） days of pseudo－pregnancy was collected. An in vivo artificial decidualization model was established and mouse endometrial tissues of the control side and induced side of mice were collected. An in vitro artificial decidualization model was established，and the mouse endometrial stromal cells of the control group and the induced group were collected. Western blot and real-time PCR were used to measure the mRNA and protein expression of TFEB in the mouse endometrium and in mouse endometrial stromal cells. The distribution and expression of TFEB in the mouse endometrium of D5 were measured by immunohistochemistry. Results：The expression of TFEB on D4，D5，and D6 gradually decreased with increasing days of pregnancy. The mRNA and protein expression of TFEB in the endometrial IS on D5 was significantly lower than that in the IIS on D5. The mRNA and protein expression of TFEB in the endometrial tissue of the induced side was significantly lower than that in the endometrial tissue of the control side. The mRNA and protein expression of TFEB in endometrial stromal cells was significantly lower in the induced group than in the control group. Conclusion：TFEB may be involved in the endometrial decidualization in early pregnant mice，but its specific regulatory role needs to be further explored.