Objective:To protect the first-line medical staff who have been fighting against Coronavirus Disease 2019(COVID-19) by taking effective measures. Methods:Causes of infection among medical staff were analyzed,and a series of protection,disinfection and isolation measures were formulated on the basis of finding problems and summing up experiences or learning from lessons carefully. Results:Through comprehensive protective measures,there was no infection among the 328 Chongqing medical team members who have been fighting in Hubei Province. Conclusion:In front of the outbreak of COVID-19,we should take the disease seriously with a scientific attitude and strengthen the consciousness of self-protection. On the basis of all kinds of physical barriers,front-line medical staff should establish behavioral barriers,and implement standard prevention strictly,so as to effectively prevent medical staff from COVID-19.