胃癌中IBSP基因表达及预后的生物信息学分析

Bioinformatics analysis of IBSP gene expression and prognosis in gastric cancer

摘要:

目的：通过生物信息学分析筛选出胃癌和正常胃黏膜间差异表达基因，分析并预测其在胃癌发生发展及预后中的价值。方法：从癌症基因组图谱（the Cancer Genome Atlas，TCGA）数据库下载胃癌患者RNA-seq数据，使用软件R-Studio筛选差异表达基因，运用DAVID进行基因本体（gene ontology，GO）富集分析和京都基因与基因百科全书（Kyoto encyclopedia of genes and genomes，KEGG）通路分析，研究PI3K-Akt通路中表达差异最明显的基因，通过网站UALCAN对其进行表达量及临床病理特征分析，利用在线网站STRING构建蛋白互作网络，同时利用Kaplan-Meier Plotter在线分析其与胃癌患者预后的相关性。结果：共获得5 704个差异表达基因，包括1 225个上调和1 479个下调的蛋白编码基因；KEGG通路分析确定骨涎蛋白（inte－grin binding sialoprotein，IBSP）为目的基因；IBSP基因在胃癌组织中明显高表达（P<0.001）；且与胃癌患者的临床病理特征及不良预后明显相关（P<0.05）。结论：IBSP基因作为胃癌中潜在的癌基因，可能通过PI3K-Akt信号通路调控胃癌的早期进展，进而导致胃癌患者预后不良，有望成为胃癌新的临床诊断和预后标志物。

Abstract:

Objective：To screen differentially expressed genes between gastric cancer and normal gastric mucosa by bioinformatics analysis；to analyze and predict its value in the development and prognosis of gastric cancer. Methods：RNA-seq data from gastric cancer patients were downloaded from the Cancer Genome Atlas（TCGA） database，and differentially expressed genes were screened using the software R-Studio. The DAVID database was used to perform genetic ontology（GO） enrichment analysis and Kyoto Ency－clopedia of Genes and Genomes（KEGG） pathway analysis for differentially expressed genes. The gene with the most significant differ－ence in expression in the PI3K-Akt signaling pathway was identified for further study：its expression level and clinicopathological were analyzed using UALCAN，the website STRING was used to construct its protein-protein interaction networks，and Kaplan-Meier Plotter was used to analyze its correlation with the prognosis of gastric cancer patients. Results：A total of 5704 differentially expressed genes were obtained，including 1225 up-regulated and 1479 down-regulated protein-coding genes；KEGG pathway analysis identified integrin binding sialoprotein（IBSP） as the key gene. IBSP was highly expressed in gastric cancer tissues（P<0.001），which was signif－icantly associated with the clinicopathological features and poor prognosis in patients with gastric cancer（P<0.05）. Conclusion：As a potential oncogene in gastric cancer，IBSP may regulate the early progress of gastric cancer through PI3K-Akt signaling pathway and lead to poor prognosis of gastric cancer patients，and it is expected to become a new clinical diagnosis and prog－nostic marker for gastric cancer.