胃腺癌中SIX6的表达及对预后判断价值的研究

Expression and significance of sine oculis homeobox homolog-6 in patients with gastric adenocarcinoma

摘要:

目的：检测胃腺癌中同源异型蛋白-6（sine oculis homeobox homolog-6，SIX6）的表达，分析其对预后判断的价值。方法：本实验选择79例胃腺癌作为观察组，选择对应的标本中距肿物边缘>3 cm的正常胃黏膜组织79例作为对照组，均留取新鲜组织及石蜡包埋组织，应用免疫组化法检测2组石蜡包埋组织中SIX6蛋白的表达，应用Western blot检测2组新鲜标本中SIX6蛋白的半定量表达，应用生存分析法观察SIX6与生存时间的关系。应用实时荧光定量PCR法检测15例胃腺癌和正常胃黏膜组织中SIX6 mRNA的表达。结果：2组中SIX6表达的阳性率差别有统计学意义（53.2% vs. 6.3%， χ2=41.461，P=0.000）。观察组中SIX6的表达与肿瘤的最大径（33.3% vs. 69.8%， χ2=10.446，P=0.001）、浸润深度（21.9% vs. 74.5%， χ2=21.149，P=0.000）、脉管癌栓（44.0% vs. 69.0%，χ2=4.594，P=0.032）、淋巴结转移（31.8% vs. 80.0%， χ2=18.175，P=0.000）和炎细胞浸润程度（25.9% vs. 67.3%， χ2=12.223，P=0.000）有关，而与性别、年龄和分化程度无明显相关性（P>0.05）。生存分析显示观察组中SIX6的表达与生存时间相关（ χ2=4.243，P=0.040）。胃腺癌中SIX6蛋白的半定量表达（1.21±0.06 vs. 0.92±0.04，t=3.85，P=0.000）和mRNA（1.30±0.18 vs. 0.82±0.11，t=2.25，P=0.033）的表达量明显高于正常胃黏膜组织。结论：SIX6蛋白和mRNA在胃腺癌组织中表达明显升高，对肿瘤的形成和发展有一定的调节作用。SIX6的表达与预后有关。

Abstract:

Objective：To detect the expression of sine oculis homeobox homolog-6 （SIX6） in gastric adenocarcinoma，and analyze its value in judging prognosis. Methods：A total of 79 cases of gastric adenocarcinoma tissue were collected as the observation group，while another 79 cases of normal gastric mucosa tissue with a distance > 3 cm to tumor boundary were collected as the control group. All cases were took the fresh tissue and conducted the paraffin-embedded tissue. Immunohistochemisty was used to detect the expres－sion of SIX6 protein in paraffin-embedded tissue，Western blot was used to detect the semiquantative expression of SIX6 protein in the fresh tissue，survival analysis was used to observe the relationship between SIX6 and survival time，and real-time fluorescence quantitative PCR was used to detect the expression of SIX6 mRNA in 15 cases gastric adenocarcinom tissue and 15 cases of normal gastric mucosa tissue. Results：Positive rate of SIX6 expression in two groups had statistically significant differences in two groups （53.2% vs. 6.3%， χ2=41.461，P=0.000）. In the experimental group，expression of SIX6 was correlated with tumors' maximum diameter（33.3% vs. 69.8%， χ2=10.446，P=0.001），infiltrated depth（21.9% vs. 74.5%， χ2=21.149，P=0.000），vascular thrombus（44.0% vs. 69.0%， χ2=4.594，P=0.032），lymph node metastasis（31.8% vs. 80.0%， χ2=18.175，P=0.000） and inflammatory cell infiltration（25.9% vs. 67.3%， χ2=12.223，P=0.000），but not with gender，age and cell differentiation（P>0.05）. Survival analysis results showed that expression of SIX6 was correlated with survival time（ χ2=4.243，P=0.040）. Semiquantative expression of SIX6 protein（1.21±0.06 vs. 0.92±0.04，t=3.85，P=0.000） and mRNA（1.30±0.18 vs. 0.82±0.11，t=2.25，P=0.033） in the experimental group were higher than those in the control group. Conclusion：Higher expressions of SIX6 protein and mRNA in the experimental group are significantly increased in the gastric adenocarcinoma tissue，which plays a certain adjusting role in the formation and development of tu－mor. SIX6 is correlated with the prognosis of gastric adenocarci－noma.