LncPANDAR通过靶向miR-637调控甲状腺癌增殖、转移的机制研究

Long-chain non-coding PANDAR regulates proliferation and metastasis of thyroid carcinoma by targeting miR-637

摘要:

目的：探究长链非编码RNA PANDAR（long-chain non-coding RNA PANDAR，PANDAR）促进甲状腺癌生长转移的分子机制。方法：首先运用实时荧光定量PCR测定甲状腺癌患者血清和癌组织中PANDAR表达情况，再通过MTT、流式、Tran－swell等方法测定PANDAR对甲状腺癌细胞生长、凋亡、迁移能力的影响，通过双荧光素酶报告基因实验确定PANDAR的作用靶点为miR-637，最后再测定miR-637在PANDAR对甲状腺癌细胞生长、凋亡和迁移能力中的作用。结果：PANDAR在甲状腺癌患者血清和癌组织中表达明显升高，且PANDAR能明显促进细胞生长和迁移，并抑制细胞凋亡，且PANDAR的作用靶点为miR-637，miR-637能逆转PANDAR诱导的细胞增殖、转移和凋亡减少。结论：PANDAR通过靶向miR-637调节甲状腺癌的增殖和转移。

Abstract:

Objective：To explore the molecular mechanism of long-chain non-coding RNA PANDAR（LncRNA PANDAR） to promote the growth and metastasis of thyroid cancer. Methods：Firstly，real-time quantitative PCR was used to detect the expression of PANDAR in serum and cancer tissues of patients with thyroid cancer；measures like MTT，flow cytometry and Transwell were used to detect the effect of PANDAR on growth，apoptosis and migration of thyroid cancer cells. The dual luciferase reporter gene assay was used to determine that miR-637 was the target of PANDAR and to determine the role of miR-637 in PANDAR on growth，apoptosis and mi－gration of thyroid cancer cells. Results：Expression of PANDAR was significantly increased in serum and cancer tissues of patients with thyroid cancer，and PANDAR was able to significantly promote cell growth and migration，and inhibit apoptosis. The target of PANDAR was miR-637 and miR-637 was able to reverse PANDAR-induced cell proliferation，metastasis and apoptosis. Conclusion：PANDAR regulates proliferation and metastasis of thyroid cancer by targeting miR-637.