GNG4、TIMP1在结肠癌诊断及预后价值的生物信息学分析

Bioinformatics analysis of GNG4 and TIMP1 on diagnostic and prognosis significance in colon cancer

摘要:

目的：通过生物信息学方法，筛选可能与结肠癌诊断及预后相关的基因。方法：从基因表达共享数据库（the expression omnbus，GEO）下载结肠癌相关基因芯片GSE37364、GSE41328的cRNA表达谱数据集并筛选差异表达基因（differentially ex－pressed genes，DEGs），分别通过STRING在线数据库、Cytoscape软件、GEPIA在线工具及Metascape在线软件进行DEGs的蛋白互作（protein-protein-interactions，PPI）网络分析、核心基因筛选、预后分析、表达水平验证及通路分析。结果：鉴定出核心基因18个（THY1、BMP4、CD44、CXCL8、GNG4、COL1A2、IL1B、SERPINE1、IGF1、SPP1、COL1A1、HGF、POSTN、TIMP1、CXCL12、GCG、IL6、PTGS2），其中GNG4和TIMP1的高表达可能与预后不良相关，并参与PI3K-AKT、HIF-1、趋化因子等信号通路的调节。结论：本研究为结肠癌诊断及预后研究提供了潜在靶基因，也为解析其分子调控机制提供了线索。

Abstract:

Objective：To screen the gene that may be associated with diagnosis and prognosis of colon cancer through bioinformatics. Methods：The cRNA expression profiles data set of GSE37364 and GSE41328 were obtained from the expression omnbus（GEO） and the differentially expressed genes（DEGs） between colon adenocarcinoma tissues and normal colon tissues were screened. STRING online database，Cytoscape，GEPIA online tool and Metascape online software were used to perform the DEG-associated protein-pro－tein-interactions（PPI） network analysis，to screen the hub gene，to perform the prognosis analysis and to perform the pathway analysis，respectively. Results：A total of 18 hub genes were identified（THY1，BMP4，CD44，CXCL8，GNG4，COL1A2，IL1B，SERPINE1，IGF1，SPP1，COL1A1，HGF，POSTN，TIMP1，CXCL12，GCG，IL6 and PTGS2），among which high expression of GNG4 and TIMP1 was pos－sible to have association with poor survival and participate in the regulation of PI3K-AKT，HIF-1 and chemokine. Conclusion：Our study provides some potential target genes for the diagnosis and prognosis of colon cancer and gives a clue to molecular regulation mechanism.