应用蛋白质组学技术探索鲍曼不动杆菌感染血浆生物标志物

Exploration on plasma biomarkers of Acinetobacter baumannii infection based on proteomics techniques

摘要:

目的：利用蛋白质组学技术探索鲍曼不动杆菌（Acinetobacter baumannii，A. baumannii）感染的血浆生物标志物。方法：采集血流感染患者的血浆，分为A.baumannii感染组（n=16）、其他G-菌感染组[8例肺炎克雷伯菌（Klebsiella pneumoniae，K.pneumoniae）感染、8例大肠杆菌（Escherichia coli，E. coli）感染]。另收集16例健康人的血浆。对上述受试者的血浆蛋白进行质谱检测及生信分析，筛选出与A.baumannii感染相关的差异蛋白。结果：A.baumannii感染者区别于其他G-菌感染者的蛋白共13个，区别于健康人的共52个，同时区别的共3个（CNDP1、IGLL5、PEDF，均P＜0.05）。GO功能分析提示这些差异蛋白多集中在胞外区，主要参与凝血、炎症、免疫反应，KEGG分析显示参与补体和凝血瀑布反应通路的蛋白最多。结论：A. baumannii感染者血浆蛋白质组的变化与凝血、炎症、免疫反应密切相关。CNDP1、IGLL5、PEDF可能成为诊断A. baumannii感染的候选血浆标志物。

Abstract:

Objective：To find the plasma biomarkers of Acinetobacter baumannii（A. baumannii） infection based on proteomics tech－niques. Methods：Plasma of patients with blood stream infection was collected，and was divided into A.baumannii infection group（n=16），other G- bacteriainfection group（8 cases of K. pneumoniae infection and 8 cases of E. coli infection），and plasma of other 16 healthy people（control group） was collected. The plasma proteins of the above subjects were analyzed by mass spectrometry and bioinformatics to find the specific proteins related to A. baumannii infection. Results：There were 13 proteins in A. baumannii infection which were different from other G- bacteria infections，52 proteins were different from health controls，and 3 differential proteins in the same time（CNDP1，IGLL5，PEDF，all P<0.05）. Data from the GO analysis showed that most differential proteins were in the extracellular region，which were mostly related to coagulation，inflammation and immune response，while KEGG analysis showed that most proteins were involved in the complement and clotting cascade pathway. Conclusion：Changes in plasma proteome of A. baumannii infection were closely related to inflammation，coagulation and immune response. CNDP1，IGLL5 and PEDF may be candidate plasma biomarkers for A. baumannii infection.