急性髓系白血病患者早期血清CCN1蛋白检测及临床价值

Detection and clinical significance of serum CCN1 proteinin in patients with early acute myeloid leukemia

摘要:

目的：研究CCN1蛋白在急性髓系白血病（acute myeloid leukemia，AML）患者早期血清中的表达水平及其与临床特征的相关性，探讨CCN1在早期急性髓系白血病中的诊断价值。方法：收集96例急性髓系白血病早期患者和159名同年龄段、性别匹配的健康对照者血清样本，采用ELISA检测血清中的CCN1蛋白水平，通过ROC曲线评估其作为诊断标志物的价值；比较CCN1表达水平与临床特征的相关性；分析不同AML分型分组中的血清CCN1表达是否具有差异。结果：AML早期患者血清中的CCN1表达水平明显低于健康对照组（P=0.000），ROC曲线下面积为0.823，最佳cut-off值是101.22 pg/mL，灵敏度为0.823，特异度为0.667。CCN1表达水平与临床特征无相关性。AML不同分型与对照组均有统计学差异（P=0.000），但AML各型之间的CCN1差异无统计学意义（P >0.05）。结论：CCN1蛋白在急性髓系白血病早期患者和正常人血清中表达差异显著，因此CCN1在急性髓系白血病早期诊断中可能有诊断价值。

Abstract:

Objective：To investigate correlation between the serum concentrations of CCN1 protein and its clinical features，and to explore the diagnostic value of CCN1 in early acute myeloid leukemia（AML） patients. Methods：Sandwich enzyme-linked immunosor－bent assay（ELISA） was applied for detecting the concentrations of serum CCN1 protein in 96 early AML patients and 159 control healthy people. ROC curve was used to assess the diagnostic value of CCN1 in early AML patients. The concentrations of serum CCN1 between the distinct clinical features groups and the classification groups were compared. Results：The concentrations of serum CCN1 protein in 96 early AML patients were significantly lower than those in healthy controls（P=0.000）. The area under the ROC curve was 0.823，and the optimal cut-off value was 101.22 pg/mL（sensitivity=0.823，specificity=0.667）. The concentrations of serum CCN1 protein had no correlation with clinical features. There were significant differences between different AML classification types and the control groups（P=0.000），but no significant difference was observed among CCN1 of AML types（P >0.05）. Conclusion：The concentrations of serum CCN1 protein in early AML patients are significantly different from those in control groups，which suggests that serum CCN1 protein concentration is a biomarker with broad application prospect for early AML diagnosis.