基于纸基微流控的核酸分子快速检测技术研究进展

Progress in rapid detection of nucleic acid molecules by integrated paper-based microfluidics

摘要:

核酸分子具有丰富的遗传信息，对疾病诊断具有关键价值。传统核酸检测技术受限于耗时长、需大型仪器设备等局限，严重制约了其在大规模、突发性疾病检测中的应用。纸基微流控具有便携、自驱动等优势，在整合等温扩增技术后，为核酸分子快速、准确的检测提供可能。本文从纸基微流控的设计出发，综述纸基微流控在核酸分子检测中的研究进展，分析影响其检测灵敏度、稳定性等的干扰因素，为推动纸基微流控向临床转化提供重要参考。

Abstract:

Nucleic acid is of great importance to disease diagnosis because of its rich genetic information. Traditional nucleic acid detection technology is limited by the limitation of time consuming and large equipment， which seriously restricts its application in large-scale and sudden disease detection. Paper-based microfluidic technology has the advantages of portability and self-actuation， providing the possibility for rapid and accurate detection of nucleic acid molecules after integrating isothermal amplification technology. Based on the design of paper-based microfluidic technology，this review has summarized the research progress of paper-based microfluidic technology in the detection of nucleic acid molecules，and analyzed the interference factors that affect the sensitivity and stability of the detection， providing an important reference for promoting the clinical transformation of paper-based microfluidic technology.