探讨嗜碱性粒细胞CD63、CD203c、FcεRI在哮喘-慢性阻塞性肺疾病重叠的表达

Investigation on the expression of basophil CD63, CD203C and FcεRI in the overlap of asthma and chronic obstructive pulmonary disease

摘要:

目的： 通过对哮喘-慢性阻塞性肺疾病重叠（asthma-chronic obstructive pulmonary disease overlap，ACO）患者急性期与缓解期嗜碱性粒细胞表面分子表达水平的测量比较，探讨其与病情的关系。方法： 招募健康人32例及收集在锦州医科大学附属第三医院治疗的ACO患者急性期及缓解期各14例，采集肘部静脉血，使用流式细胞术应用3种设门策略获得嗜碱性粒细胞，比较不同时期患者嗜碱性粒细胞表面分子（CD63、CD203c、FcεRI）表达水平与健康组的差异，并对结果和疾病状态进行回顾性研究。结果： 急性期嗜碱性粒细胞CD63平均荧光强度（Mean fluorescence intensity，MFI）较健康组有统计学差异（P<0.05），急性期CD203c、FcεRI MFI较健康组无统计学差异；缓解期CD63、FcεRI MFI较健康组有统计学差异（P<0.05），缓解期CD203c MFI较健康组无统计学差异（P>0.05），急性期与缓解期比较，嗜碱性粒细胞CD63、CD203c、FcεRI MFI没有统计学差异。急性期、缓解期CCR3⁺、CCR3⁺/CD123⁺HLA-DR^-、CD123⁺HLA-DR^- MFI较健康组有统计学差异（P<0.001）。结论： ACO患者嗜碱性粒细胞表面分子CD63、FcεRI表达增强，这表明嗜碱性粒细胞参与了ACO的发病，通过检测其表面分子表达量对ACO病情的判断有提示意义。

Abstract:

Objective: To investigate the relationship between the expression level of basophil surface molecules in patients with asthma-chronic obstructive pulmonary disease overlap (ACO) in acute period and in remission period. Methods: Totally 32 healthy people and respectively 14 cases of acute and remission ACO patients admitted to The Third Affiliated Hospital of Jinzhou Medical University were collected in the study. By collecting the venous blood from the elbow, the basophils were obtained by flow cytometry using three gating strategies. In order to compare the expression levels of basophil surface molecules (CD63, CD203c, FcεRI) between patients and healthy groups at different stages, the results and disease states were retrospectively studied. Results: The Mean fluorescence intensity (MFI) of CD63 in acute basophil was significantly different from that in healthy group (P<0.05), and there was no significant difference in CD203c and FcεRI in acute basophil compared with that in healthy group. The MFI of CD63 and FcεRI in the remission phase was significantly different from that in the healthy group (P<0.05), and the MFI of CD203c in the remission phase was not significantly different from that in the healthy group (P>0.05). There were no significant differences in the MFI of CD63, CD203c and FcεRI in the basophil between the acute phase and the remission phase. The MFI of CCR3⁺, CCR3⁺/CD123⁺HLA-DR^- and CD123⁺HLA-DR^- in acute and remission stages was significantly different from that in healthy groups (P<0.001). Conclusion: The expression of CD63 and FcεRI on the surface of basophil in the ACO patients is enhanced, suggesting that basophil is involved in the pathogenesis of ACO, and the detection of its surface molecular expression is suggestive to the judgment of ACO condition.