当归芍药散通过调节Sirt1/NF-κB信号通路改善高脂饮食诱导大鼠非酒精性脂肪肝炎症反应

Danggui Shaoyao Powder improves inflammatory response in rats with nonalcoholic fatty liver disease induced by high-fat diet by regulating the Sirt1/NF-κB signaling pathway

摘要:

目的 研究当归芍药散治疗高脂饮食（high-fat diet，HFD）诱导的大鼠非酒精性脂肪性肝病（nonalcoholic fatty liver disease，NAFLD）炎症反应的作用机制。方法 将大鼠随机分为5组：正常组、HFD组、阿伐他丁组、当归芍药散低剂量组、当归芍药散高剂量组。大鼠给予高脂肪饲料（59.3%脂肪饲料）6周，正常组大鼠同时给予正常饲料。从第5周开始，阿伐他丁组大鼠灌胃给予25 mg/kg阿伐他丁2周，当归芍药散低剂量组大鼠灌胃给予12.9 g/kg当归芍药散2周，当归芍药散高剂量组大鼠灌胃给予25.8 g/kg当归芍药散2周，检测大鼠血清丙氨酸氨基转移酶（alanine aminotransferase，ALT）、天冬氨酸氨基转移酶（aspartate aminotransferase，AST）、甘油三酯（triglyceride，TG）、胆固醇（cholesterol，TC）、血清细胞因子水平。同时检测大鼠肝脏沉默信息调节因子1（sirtuin 1，Sirt1）/核转录因子-κB（nuclear factor kappa-B，NF-κB）信号通路蛋白表达。结果 与模型组比较，当归芍药散能显著降低血清ALT、AST、TG、TC、血清细胞因子水平，改善肝脏组织病理学改变。此外，显著增加大鼠肝脏Sirt1表达，抑制肝脏NF-κB蛋白表达。结论 当归芍药散通过Sirt1/NF-κB通路治疗HFD大鼠NAFLD。

Abstract:

Objective To investigate the mechanism of action of Danggui Shaoyao Powder in the treatment of inflammatory response in rats with nonalcoholic fatty liver disease（NAFLD） induced by high-fat diet（HFD）.Methods Rats were randomly divided into normal group，HFD group， avastatin group，Danggui Shaoyao Powder low-dose group，and Danggui Shaoyao Powder high-dose group. The rats were given HFD（containing 59.3% fat） for 6 weeks，while the rats in the normal group were given normal diet. Since week 5，rats in the avastatin group were given 25 mg/kg of Avastatin by gavage for 2 weeks， while rats in the low-dose Danggui Shaoyao powder group were given 12.9 g/kg of Danggui Shaoyao powder by gavage for 2 weeks， and rats in the high-dose Danggui Shaoyao powder group were given 25.8 g/kg of Danggui Shaoyao powder by gavage for 2 weeks，and the serum levels of alanine aminotransferase（ALT），aspartate aminotransferase（AST），triglyceride（TG），cholesterol（TC），and cytokines were measured. Meanwhile，the expression levels of the sirtuin 1（Sirt1）/nuclear factor kappa-B（NF-κB） signaling pathway proteins in rat liver were also measured.Results Compared with the model group，Danggui Shaoyao Powder significantly reduced the serum levels of ALT，AST，TG，TC，and cytokines and improved the pathological changes of the liver. In addition，it significantly increased the expression of Sirt1 and inhibited the expression of NF-κB in rat liver.Conclusion Danggui Shaoyao Powder exerts a therapeutic effect on rats with NAFLD induced by HFD through the Sirt1/NF-κB pathway.