多光谱光声层析成像技术在精索静脉曲张大鼠睾丸成像中的初步探索

Initial exploration of multispectral optoacoustic tomography in imaging of the testes in varicocele rats

摘要:

目的 探索光声层析成像（photoacoustic tomography，PAT）和多光谱光声层析成像（multispectral optoacoustic tomography，MSOT）在精索静脉曲张大鼠模型中进行睾丸结构和功能成像的可行性。方法 将18只SD大鼠随机分为3组（空白组、模型组、迈之灵组），每组6只。模型组、迈之灵组均采用缩窄左肾静脉并结扎左侧精索静脉侧支方法造模，经过4周相应措施干预后，比较实验大鼠左侧睾丸的PAT/MSOT图像、氧代谢及氧化应激水平，研究不同干预措施实验大鼠的睾丸成像特征。结果 PAT/MSOT能对大鼠睾丸结构进行清晰成像，能够反映睾丸内的氧代谢水平。结论 PAT/MSOT可检测精索静脉曲张引起的睾丸结构和功能改变，是一种简易、无创、安全的新型睾丸评估方法。

Abstract:

Objective To explore the feasibility of photoacoustic tomography（PAT） and multispectral optoacoustic tomography（MSOT） for testicular structure and function imaging in a varicocele rat model.Methods Eighteen SD rats were randomly divided into blank group，model group，and Aescuven forte group，with 6 rats in each group. In the model group and Aescuven forte group，a varicocele rat model was established by narrowing the left renal vein and ligating the left spermatic vein. After 4 weeks of intervention，the PAT/MSOT image，oxygen metabolism，and oxidative stress levels of rat left testicle were compared to investigate the testicular imaging characteristics of rats with different interventions.Results PAT/MSOT provided a clear image of rat testicular structure and reflected the level of oxygen metabolism in the testes.Conclusion PAT/MSOT can be used to detect the structural and functional changes in the testis caused by varicocele. PAT/MSOT is a novel，simple，and safe method for non-invasive evaluation of testes.