超高速细胞分选平台结合cDNA microarray技术筛查宫颈癌细胞潜在分子标志物

Screening of potential molecular markers in cervical cancer cells by ultra-high-speed cell selection platform combined with cDNA microarray technology

摘要:

目的： 通过超高速细胞分选平台结合cDNA microarray技术，筛查宫颈癌细胞可能潜在的分子标志物。方法： 采用MoFlo XDP型超高速细胞分选平台纯化细胞膜表面表达CD38和不表达CD38的宫颈癌细胞，利用RNAlater技术得到cDNA microarray实验所需RNA，然后进行基因芯片分析。结果： 利用MoFlo XDP型超高速细胞分选平台可以获得纯度为99.0%以上的CD38阳性表达宫颈癌细胞。结论： cDNA microarray分析发现了RORA、PLIN4、AUTS2、IFITM1等宫颈癌细胞潜在分子标志物，为宫颈癌研究提供了新的技术方法。

Abstract:

Objective: To screen potential molecular markers of cervical cancer cells by ultra-high speed cell sorting platform combined with cDNA microarray technology. Methods: In this paper, the MoFlo XDP-type ultra-high-speed cell selection platform was used to purify cervical cancer cells expressing CD38 and not expressing CD38 on the cell membrane surface. RNAlater technology was used to obtain the RNA required for cDNA microarray experiments, and then by gene chip analysis was made. Results: The MoFlo XDP-type ultra-high-speed cell selection platform can be used to obtain cervical cancer cells with a CD38-positive expression of 99.0% purity. Conclusion: Through the analysis of cDNA microarray, potential molecular markers of cervical cancer cells such as RORA, PLIN4, AUTS2, and IFITM1 have been found, which provides a new technical method for the research of cervical cancer.