Abstract:Objective: To investigate the expression and significance of Fibrillin-1, Fibulin-5 and lysyl-oxidase-1 (LOXL-1) on the anterior vaginal wall in patients with pelvic floor organ prolapse. Methods: A total of 108 patients with pelvic floor organ prolapse who underwent surgery in our hospital from January 2018 to February 2019 were selected as the observation group, including 67 patients with POP-Q ofⅡdegree and 41 patients with POP-Q ofⅢdegree. At the same time, 92 patients with cervical intraepithelial neoplasia or gynecological benign lesions who underwent total hysterectomy were selected as the control group. The tissue of anterior vaginal wall was taken. The expression of Fibrillin-1, Fibulin-5 and LOXL-1 was detected by immunohistochemistry; the expression level of Fibrillin-1, Fibulin-5 and LOXL-1 mRNA in the tissue samples were quantitatively measured by RT-PCR, and then were compared and analyzed. Meanwhile, the relationship of Fibrillin-1, Fibulin-5 and LOXL-1 was analyzed. Results: The positive expression rate of Fibrillin-1, Fibulin-5 and LOXL-1 in the observation group was significantly lower than that in the control group, and the difference had statistical significance according to chi-square test and t test (all P=0.000). The positive expression rate Fibrillin-1, Fibulin-5 and LOXL-1 protein and the relative expression of mRNA in patients with POP-Q ofⅡdegree were higher than those in patients with POP-Q ofⅢdegree, and the difference had statistical significance by chi-square test and t test (P=0.031, P=0.017, P=0.008, P=0.000, P=0.000, P=0.013, respectively). The expression of Fibrillin-1 and Fibulin-5, Fibrillin-1 and LOXL-1, and Fibulin-5 and LOXL-1, and relative mRNA expression in the anterior vaginal wall of POP patients were significantly positively correlated according to Spearman rank correlation and Pearson correlation (all P=0.000). Conclusion: The expression of Fibrillin-1, Fibulin-5 and LOXL-1 in vaginal tissues of patients with pelvic floor organ prolapse is decreased, which is closely related to the degree of prolapse, and the correlation among them is significant through the cross linking.
