Abstract:Objective: To identify the expression of RNA hsa_circ_0066336 (circFLNB) in bone marrow-derived cells, and to analyze its potential function. Methods: Bone marrow-derived cells THP-1 and Jurket were selected as model cells. Divergent primers were designed, and differences of circRNA expression in two model cells were analyzed. T-A cloning was used to construct plasmids for comparing. Then Arraystar software was used to predict the target miRNA of hsa_circ_0066336. The target miRNA was extracted and reverse transcribed from THP-1, and then was detected by Real-time PCR. Results: ①CircFLNB was successfully amplified in THP-1 and Jurket cells, and statistical analysis showed that their expression was not significantly different in two model cells (P=0.105). ②The plasmid containing hsa_circ_0066336 was cloned, and a back-splice junction was found after comparing two sequences.③Five target miRNAs of hsa_circ_0066336 were predicted by Arraystar software and corresponding expressions were detected. Conclusion: CircFLNB is a circular RNA expressed in bone marrow derived cells and it is suggested that circFLNB may involve in the development of various diseases by comprehending its downstream miRNA function.
