Abstract:Objective: To observe the influence of proanthocyanidins (OPC) on serum cardiac troponinⅠ (cTn-Ⅰ), superoxide dismutase (SOD), malondialdehyde (MDA), glutathione peroxidase (GSH-px) on the expression level of RIHD rats, as wells as their possible effects and mechanism of action. Methods: Sixty male sprague dawley (SD) rats were randomized into 6 groups, with 10 rats in each group, namely normal control group, simple irradiation group, OPC low dose (50 mg/kg) group, OPC middle dose (100 mg/kg) group, OPC high-dose (150 mg/kg) group, and blueberry (200 mg/kg) group. In addition to the normal control group, the rats in the other groups were given 6 MV X-ray single high-dose irradiation to the heart, with a total dose (Dt) of 20 Gy, and the rats in the OPC group and blueberry group were intragastrically administered the corresponding drugs. Four weeks after the radiotherapy, hematoxylin eosin (HE) staining and Masson staining were used to observe the pathological changes of myocardial tissue in rats, and enzyme-linked immunosorbent assay (ELISA) was used to detect the expression levels of serum cTn-Ⅰ, SOD, MDA, GSH-px. Results: Compared with the normal control group, the pathological scores and myocardial collagen volume fraction (CVF) scores of the simple irradiationgroup, the OPC group, and the blueberry group increased (P<0.05); compared with the simple irradiation group, the pathological scores and CVF scores of the OPC group and the blueberry group increased (P<0.05), with significant differences. Compared with the normal control group, the expression levels of cTn-Ⅰand MDA in the simple irradiation group, OPC group, and blueberry group increased, while the expression levels of SOD and GSH-px decreased; compared with the simple irradiation group, the expression levels of cTn-Ⅰand MDA in the OPC group and blueberry group increased, while the expression levels of SOD and GSH-px decreased, with significant differences (P<0.05). Conclusion: OPC can inhibit the oxidative stress, down-regulate the expression levels of serum cTn-Ⅰand MDA in RIHD rats, and up-regulate the expression levels of serum SOD and GSH-px, thus inhibiting the myocardial damage in RIHD rats, with a better curative effect than the blueberry.
