Abstract:Objective: To investigate the effect of aldehyde dehydrogenase 2 (ALDH2) on monocyte (THP-1) -human aortic endothelial cells (HAECs) adhesion and its mechanisms. Methods: HAECs was divided into the Control group, ALDH2 NC group, ALDH2 group, siRNA-ALDH2 NC group, siRNA-ALDH2-1, -2, -3 groups. The expression of ALDH2 protein and mRNA was detected by Western blot and real-time PCR. The adhesive ability between THP-1 and HAECs was detected by cell adhesion assay. The transcription of nuclear factor-kappa B (NF-κB) was detected by Western blot and immunofluorescence. Results: Compared with the Control group and the ALDH2 NC group, the expression of ALDH2 was up-regulated in the ALDH2 group (P<0.05). Compared with the Control group and the siRNA-ALDH2 NC group, the expression of ALDH2 was down-regulated in the siRNA-ALDH2-1, -2, -3 groups (P<0.05), and the expression of ALDH2 was the lowest in the siRNA-ALDH2-2 group. Compared with Control group and ALDH2 NC group, cell adhesive ability, the expression of NF-κB in nucleus and the red fluorescence was decreased (P<0.05), and the expression of NF-κB in cytoplasm increased in ALDH2 group (P<0.05). Compared with Control group and siRNA-ALDH2 NC group, cell adhesive ability, the expression of NF-κB in nucleus and the red fluorescence was increased (P<0.05), the expression of NF-κB in cytoplasm decreased in siRNA-ALDH2 group (P<0.05). Conclusion: ALDH2 could inhibit the adhesion between THP-1-HAECs by inhibiting NF-κB nuclear transcription, and finally alleviate atherosclerosis.
