Abstract:Objective: To investigate the effect of melatonin (Mel) on the JAK2/STAT3 signaling pathway of hepatic stellate cell-T6 (HSC-T6) induced by platelet-derived growth factor (PDGF). Methods: HSC-T6 cells were divided into 6 groups: control group, model group, experimental groups (Mel 1 nmol/L, Mel 1 μmol/L, Mel 0.1 mmol/L) and inhibitor group (AG490 JAK2/STAT3 pathway inhibitor). Real-time fluorescent polymerase chain reaction (RT-PCR) was used to detect the mRNA expression of α-smooth muscle actin (α-SMA) and collagen typeⅠ (collagen-Ⅰ) in HSC-T6, and the protein expression detection of JAK-2, P-JAK2 and STAT3, P-STAT3 proteins in HSC-T6 cells was made by immunohistochemistry. Results: Compared with the control group, PDGF significantly activated the proliferation of HSC-T6, and significantly up-regulated the expression of α-SMA, collagen-ⅠmRNA and JAK-2, P-JAK2, STAT3 and P-STAT3 in HSC-T6.Compared with model group, Mel and JAK2/STAT3 pathway inhibitor inhibited PDGF induced HSC-T6 proliferation, and significantly down-regulated the expressions of α-SMA, collagen-ⅠmRNA and JAK-2, P-JAK2, STAT3 and P-STAT3.Conclusion: Mel can inhibit the activation and proliferation of HSC-T6 induced by PDGF, and the mechanism may be related to the inhibition of JAK2/STAT3 signaling pathway, which may be one of the mechanisms of melatonin improving liver fibrosis.
