Abstract:Objective: To study the effect of resveratrol (RES) on left ventricular remodeling in spontaneous hypertension rat (SHR) and its role in silent information regulator 1/nuclear transcription factor-κB (SIRT1/NF-κB) signaling pathway. Methods: Eight male Wistar-Kyoto (WKY) rats aged 8 weeks were taken as the normal group (WKY group), and 40 male SHR aged 8 weeks were randomly divided into SHR group, RES group, EX527 group, RES+EX527 group, and RES+pyrrolidine dithiocarbamate (PDTC) group, with 8 rats in each group, given corresponding drug intervention for 12 weeks. The rats’weight, systolic pressure, echocardiography, left ventricular mass, left ventricular mass index (LVMI), myocardial collagen volume fraction (CVF), oxidative stress index, SIRT1 and NF-κB protein expression levels, and inflammatory factors protein expression levels were measured. And the morphological and pathological changes of the heart under HE and Masson staining were observed. Results: Compared with WKY group[LVMI (2.12±0.10) mg/g, CVF (0.18±0.09) %, SIRT1 protein expression (1.40±0.05), NF-κB protein expression (0.46±0.06)], SHR group[LVMI (3.07±0.07) mg/g, CVF (2.60±0.56) %, SIRT1 protein expression (0.78±0.08), NF-κB protein expression (1.41±0.08)] showed severe left ventricular remodeling, significantly increased levels of oxidative stress and inflammatory factors as well as NF-κB expression (P<0.05), and decreased SIRT1 expression (P<0.05). In RES group[LVMI (2.72±0.09) mg/g, CVF (1.10±0.30) %, SIRT1 protein expression (0.99±0.07), NF-κB protein expression (0.98±0.12)], compared with SHR group, left ventricular remodeling degree was reduced, oxidative stress and inflammatory factors as well as NF-κB expression levels were decreased (P<0.05), and SIRT1 expression was increased (P<0.05). The results of EX527 group[LVMI (3.18±0.08) mg/g, CVF (2.83±0.98) %, SIRT1 protein expression (0.60±0.02), NF-κB protein expression (1.68±0.07)] were opposite to those of RES group. Compared with RES group, RES+EX527 group showed a trend of reversing RES to ameliorate left ventricular remodeling. RES+PDTC group showed a tendency to further ameliorate left ventricular remodeling. Conclusion: RES can ameliorate left ventricular remodeling of SHR, and its mechanism may be related to regulation of SIRT1/NF-κB pathway to reduce oxidative stress and inflammatory response damage.
