Damage modes of hepatocytes after bilateral nephrectomy in rats

Abstract:

Objective：To observe the morphological changes of hepatocyte injury induced by bilateral nephrectomy due to non ischemic acute kidney injury（NIAKI）. Methods：Western blot，immunohistochemical staining，light and electron microscopy were used in this observation at 24 h after bilateral nephrectomy. Results：Acute liver damage was observed at 24 h after bilateral nephrectomy. Necrotic and vacuolization lesions were predominately evident in damaged livers after nephrectomy. Besides necrosis，hepatocytes with vac－uolization was also observed under electron microscopes. Meanwhile，polyADP-ribose polymerase-1（PARP-1），tumor necrosis factor receptor 1 and caspase-3 were expressed much stronger in NIAKI associated livers by Western blot analysis. Immunohistochemical staining demonstrated that PARP-1 positive nucleuses and caspase-3 positive cells were observed in the acute hepatic injury induced by nephrectomy. Conclusion：NIAKI can induce necrosis，apoptosis and vacuolation of liver cells，more evident in necrosis and apop－tosis，based on morphological observation. PARP mediated cell death and caspase-dependent cell death are involved in NIAKI asso－ciated hepatic damages.