Association of single nucleotide polymorphisms and combined genotypes of klotho G-395A，F352V and C370S with serum klotho protein levels in elderly people of Chinese Han population in Chongqing

Abstract:

Objective：To investigate the association between serum klotho protein level and single nucleotide polymorphisms （SNPs） and combined genotypes of Klotho G-395A，F352V and C370S in elderly people of Han nationality in Chongqing. Methods：Unrelat－ed 390 elderly individuals from Chinese Han population （in Chongqing were selected.Single nucleotide polymorphisms of Klotho gene in the G-395A，F352V and C370S were tested by allele-specific primer（ASP） PCR，and serum klotho protein level was determined by enzyme-linked immunosorbent assay（ELISA）. Results：No significant difference was found in serum klotho protein level in the aged carrying allelic genotypes of Klotho G-395A，F352V and C370S SNP（P>0.05）. According to combined genotype analysis，the klotho protein level in the elderly individuals carrying Klotho G-395G+F352F +C370S was higher than that in the elderly individuals with Klotho G-395G+F352F+C370C（P=0.056）. Conclusion：No association of allelic genotypes of Klotho G-395A，F352V and C370S SNP with serum klotho protein levels is found.But a trend is found that serum klotho protein level in those with Klotho G-395G+F352F+C370S is higher than that in the elderly individuals with Klotho G-395G+F352F+C370C.