Na+-H+ antiporter mainly expressed in the mitochondria of mammalian osteoclast

Abstract:

Objective:To investigate the effects of telmisartan on transient outward potassium currents （Ito） and L-type calcium cur－rents （ICa-L） of atrial myocardium in two kidney one clip（2K1C） rats by measuring the alteration of mRNA and protein （Kv4.2 and Cav1.2） levels in encoding genes （Kcnd2 and Cacna1c）. Methods: Totally 39 male Sprague-Dawley rats were divided into control group，2K1C and Telmisartan 5 mg/（kg·d） group，2K1C and telmisartan[10 mg/（kg·d）] group and 2K1C group.After four weeks of treatment，mRNA and protein levels of Kcnd2 and Cacna1c were assessed by reverse transcriptase polymerase chain reaction and western blot. The data were analyzed by relative quantification. Results：The expression levels of mRNA and protein of Kcnd2 were decreased{（0.23?0.02） vs. （1.0?0.12）；（0.34?0.11） vs. （0.78?0.13），P<0.01}，but those of Cacna1c were increased{（1.54?0.17）vs. （1.0?0.13）；（1.58?0.09） vs. （0.92?0.05），P<0.01} in 2K1C group compared with those in control group.The expression levels of mRNA and protein of Kcnd2 were increased{（0.32?0.01），（0.53?0.11） vs. （0.23?0.02）；（0.54?0.07），（0.72?0.08） vs. （0.34?0.11），P<0.01}，but those of Cacna1c were decreased{（1.21?0.18），（1.02?0.14） vs. （1.54?0.17）；（1.21?0.06），（0.98?0.07） vs. （1.58?0.09），P<0.01} in 2K1C and Telmisartan 5 mg/（kg·d） group as well as 2K1C and telmisartan 10 mg/（kg·d） group compared with those in 2K1C group. Conclusion：Telmisartan exerts its antiarrhythmic effects by regulating mRNA and protein levels of Kcnd2 and Cacna1c.