Effect of chronic intermittent hypoxia on oxidative stress in the myocardial muscles of rats

Abstract:

Objective：To establish the chronic intermittent hypoxia （CIH） rat animal model Dv simulation of obstructive sleep ap－nea-hypopnea syndrome（OSAHS），and to observe the effect of CIH on oxidative stress in rat myocardial injury and reoxygenation.Methods：Chronic intermittent hypoxia rat model with 16 rats were established，in which eight rats were reoxygenated after hypoxia. Glutathione（GSH） and protein carbonylation of heart muscles were determined with the method of Western blot，and then were com－pared with those of eight normal rats. Results：The glutathione of myocardial muscles were significantly decreased in chronic inter－mittent hypoxia group compared with that in control group（P<0.05）. However，the protein carbonylation levels were significantly high－er （P<0.05）. Compared with those in the control group，the glutathione and protein carbonylation levels of the reoxygenation group had no significant difference（P>0.05）. Conclusion：Oxidative stress level increase and modification of proteins takes place in myocar－dial muscle of rats with chronic intermittent hypoxia. After reoxygenation，the oxidative stress level returns to normal.