Preparation of LHRHa Paclitaxel liposomes and its anti tumor effect in vitro

Abstract:

Objective:To prepare LHRHa-Paclitaxel liposomes and to observe its inhibitory effect on ovarian cancer A2780/DDP cells in vitro. Methods:Paclitaxel-liposomes(PTX-Lipo) and LHRHa-Paclitaxel liposomes (LHRHa-PTX-Lipo) were prepared by means of film ultrasound and their morphology was examined with transmission electron microscope.The encapsulation rates of PTX-Lipo and LHRHa-PTX-Lipo were quantified with high performance liquid chromatography (HPLC).The cellular intake of NBD-Lipo and LHRHa-NBD-Lipo was embodied through detecting the intake of NBD fluorescein by ovarian cancer A2780/DDP cells with laser confocal microscope.The inhibitory effect of LHRHa-PTX-Lipo on ovarian cancer cells in vitro was measured by MTT assay and colony forming assay.Results:The mean diameter of LHRHa-PTX-Lipo particles was 123.4 nm with encapsulation rate being above 90%.The fluorescein intake of A2780/DDP cells was higher in NBD-LHRHa-Lipo group compared with that in NBD-Lipo group.The inhibitory effect of LHRHa-PTX-Lipo on the growth and clone formation of A2780/DDP cell was much more intense than that of PTX and PTX-Lipo(P<0.05).Conclusion:LHRHa-PTX-Lipo prepared by means of film ultrasound could aggregate drugs at targeted site thus increase the inhibitory effect of drugs on ovarian cancer cells in vitro.