Research on short term preservation of mouse bone mononuclear cell in the ordinary refrigerator at the temperature of 4 ℃ 

Abstract:

Objective:To find a simple and effective way of preserving bone mononuclear cells at short-term.Methods:Bone mononuclear cells from mice were isolated by density gradient centrifugation.DMEM culture solution was employed as the nutrient solution and bone mononuclear cells were preserved in the refrigerator at the temperature of 4℃.The form,recovery rate,viability and cycle of bone mononuclear cells were observed and detected optionally from the time of being preserved to 60 h after the preservation.Results:The living cell rate was over 80% and the cell recovery rate was over 70% after being preserved in the refrigerator at the temperature of 4 ℃ for two days.Furthermore,the cell cycle was similar to that of control group (P>0.05).Conclusion:Refrigerator at the temperature of 4 ℃ can safely preserve bone mononuclear cells within two days,which offers a simple and effective way of preserving.