Abstract:Objective:To investigate the effects of different temperatures on the regeneration of allogeneic nerves of rats preserved in UW solution with Tetramethylpyrazine(TMP).Methods:Totally 60 Wistar rats (male,weighing 200～250 g) were divided into five groups randomly (groups A,B,C,D and E,n=12).The sciatic nerve trunks of 18 SD rats (male,weighing 200～250 g) were incised at about 15 mm and were preserved in UW solution with TMP at different temperatures (group A:4 ℃,group B:-20 ℃,group C:-80 ℃) for fours weeks to repair the sciatic nerve defect of SD rats.Group D was allogeneic nerve graft group and Group E was autologous nerve graft group.General observation was performed at 4,8,12 weeks postoperatively while electrophysiological check,light and electron microscope check were conducted at 12 weeks postoperatively.Results:The results of A、E groups in the electrophysiological test and gastrocnemius weight test were better than those of the other groups,however,statistical differences were observed between group A and E (P＜0.05).Group A and E displayed similar results in morphology at 12 weeks after the operation.Conclusion:The regeneration of allogeneic nerves preserved in UW solution with TMP at 4 ℃ can be improved.
