Research on clinical and pathological changes of chronic hepatitis Bpatients infected with different hepatitis B virus genotypes

Abstract:

Objective:To investigate the relationship of ages,genders and nationalities in chronic hepatitis B (CHB) patients infected with different hepatitis B virus,to compare the hepatic tissue damage degree,HBV-DNA load and the liver function change among patients with different genotype.Methods:Fluorescence quantitative polymerase chain reaction (PCR) instrument was used to test HBV-DNA and restriction fragment length polymorphism (RFLP) method was used to test genotypes.Results:Among 94 patients with CHB,B genotype was accounted for 74.5% and C genotype for 25.5%.CHB patients HBV genotype had nothing to do with age and sex.The proportion of Zhuang nationality in C genotype was obviously higher than that in B genotype.The HBeAg positive rate of patients with C genotype was obviously lowered than that of patients with B genotype(P<0.05).The pathological damage degree of the hepatic tissue was correlated with B and C genotype.The proportion of C genotype in the hepatic tissue with serious inflammation and fibrosis was higher than that in B genotype(P<0.05) .The hepatic tissue damage degree was correlated with liver function indexes(P<0.05),but not HBV DNA levels. Conclusion:The HBV genotypes of enrolled CHB patients is B genotype and C genotype B genotype is mainly distributed among the population,followed by C genotype,other types or mixed types are not found yet.The patients with Han nationality is mainly B genotype and those with Zhuang nationality is primarily C genotype.Patients with C genotype have serious hepatic inflammation and fibrosis and are prone to induce servere liver damage and adverse prognosis such as liver cancer.