Correlation between single nucleotide polymorphisms of Klotho gene and metabolic syndrome in the aged

Abstract:

Objective:To investigate the correlation between the distribution of three single nucleotide polymorphisms (SNPs) of Klotho(KL) G-395A,F352V and C370S in the Klotho(KL) gene and metabolic syndrome(MS) in the elderly Han population. Methods：Totally 329 patients (aged≥60) with MS and 321 healthy people were enrolled. The single allele specific prime PCR (SASP-PCR) technique was used to detect three sites of SNPs and the distribution frequencies of allelic genotypes of the KL gene in order to analyze their relationship with MS in the aged. 〖WTB4X〗Results〖WTBZ〗: The frequency of allele AA genotype of KL G-395A in MS group was higher than that in the control group but without any significant difference (P＞0.05). According to sex stratification analysis, no significant difference in the frequency of allele AA was found either in the female subgroup or the male subgroup between the MS group and the control subgroup(P＞0.05).The frequency of allele VV genotype of KL F352V in MS group was higher than that in the control group (P＝0.053), but no significant difference of the frequency of allele VV of KL F352V was found either in the female or male subgroup between MS group and the control group according to sex stratification analysis. The frequency of allele CS genotype of KL C370S SNP in MS group was lower than that in the control group(P＜0.05). According to sex stratification analysis, the frequency of allele CS and SS in the MS male subgroup was obviously lower than that in the control subgroup(P＜0.05). Conclusion：Correlation between VV mutant genotype of KL F352V SNP and metabolic syndrome in the elderly people may be existed and the males who carried mutant genotypes of KL C370S SNP have a low risk in suffering from MS.