Abstract:Objective:To study the cancer testis (CT) gene expression in medulloblastoma of the Chinese population.Methods:Totally 25 medulloblastoma cases were collected. RT-PCR and immunohistochemical methods were used to detect medulloblastoma CT gene and protein expression respectively, combined with genotyping analysis. Results:Tumor types were statistically different between each other in non X-CT antigens(P<0.05). CT gene was expressed in at least 1 case among 25 cases. GAGE was the most commonly expressed CT gene,existing in 17 cases (68%). CT-X gene co expression was found in 19/20 cases (95%) while non X-CT gene co expression in 1/18 cases (5.6%). Among all cases, monoclonal antibody CT 7-33 (MAGEC1) was the most positive protein staining. The CT gene mRNA expression and clinical parameters (such as age and clinical outcome) were statistically analyzed, displaying no significant correlation.Conclusion：At the molecular level, CT antigen exists in at least one CT gene. Many CT genes have mRNA expression,but few have protein expression.
