Effect of siRNA mediated NFBD1 depletion on chemosensitivity in nasopharyngeal carcinoma cells

Abstract:

Objective:To investigate the effect of siRNA-mediated nuclear factor with BRCT domains protein 1(NFBD1)depletion on chemosensitivity in nasopharyngeal carcinoma cells. Methods：Using siRNA to inhibit expression of NFBD1 in nasopharyngeal carcinoma cell lines HNE-1(poorly differentiated squamous-cell carcinoma) and CNE-1(goodly differentiated squamous-cell carcinoma), RT-PCR and Western bolt analysis were used to verify the inhibitory effect of siRNA against NFBD1. MTT assay was used to determine the effect of NFBD1 depletion on the chemosensitity of nasopharyngeal carcinoma cells. Results：siRNA could specifically and efficiently deplete NFBD1 in nasopharyngeal carcinoma cells. Survival rate in the NFBD1 siRNA group was significantly degraded,compared with that in blank group and the control siRNA group. Conclusion： SiRNA-mediated NFBD1 depletion can significantly enhance the chemosensitivity of nasopharyngeal carcinoma cells to chemotherapeutic agents such as cisdiamminedichloroplatinum(CDDP).