Abstract:Objective:To observe the inhibitory effect of Curcumin on human alveolar rhabdomyosarcoma cell line PLA-802 in vitro, to detect the expressions of TGF-beta1 and Smad 4, and to explore its potential mechanisms. Methods：Human alveolar rhabdomyosarcoma cell line PLA-802 was cultured and treated with Curcumin at different concentration and at different time points. The effect of Curcumin on PLA-802 cell proliferation was studied by means of MTT; the cell cycle was detected with flow cytometry; RT-PCR and western blot were used to detect the expressions of TGF-β1 and Smad 4 at mRNA and protein levels. Results：The viability of PLA-802 cells treated with Curcumin was obviously decreased (P<0.05). Analysis of the cell cycle revealed that Curcumin induced a significant decrease in cells in the S phase and an increase in cells in the G1 phase (P<0.05). Furthermore, the expressions of TGF-β1 and its downstream factor Smad 4 at mRNA and protein levels were inhibited by Curcumin in a concentration and time dependent manner (P<0.05). Conclusion:Curcumin perhaps plays its inhibitory effects on PLA-802 cells through inhibiting the the activity of TGF-β1 signaling pathway, which will provide an important therapeutic potential for treating human aleovar rhabdomyosarcoma.
