Reno-protective effect of all-trans retinoic acid on adriamycin-induced nephropathic mice

Abstract:

Objective：To investigate the reno-protective effect of all-trans retinoic acid（ATRA） on adriamycin（ADR）-induced nephropathic mice. Methods：BALB/c mice were randomly divided into normal group，ADR-induced nephropathic group（model group）and ADR-induced nephropathy treated group（ATRA treatment group）. After ADR-induced nephropathy model was established in model and ATRA treatment group，intraperitoneal injection in normal and model group and ATRA treatment group was performed till the 10th week. Expression of Fas antigen and Fas ligand（FasL） was measured by immunohistochemistry and their mRNA expressions were detected by Real-time PCR. Results：Urinary protein excretion，serum total cholesterin（（TC），（3.09±0.89） mmol/L） and triglyc－eride（（TG），（1.33±0.29） mmol/L） were obviously increased in model group compared with those in normal group（all P<0.05）. TC and TG were （（1.27±0.31） mmol/L and （1.12±0.45） mmol/L） in ATRA treatment group，obviously increased compared with those in model group（all P<0.05）. Immunohistochemistry showed that expression levels of Fas antigen and FasL were （650.141 6±153.996 0） and （350.190 7±204.220 6） in model group，obviously higher than those in normal group，P<0.05. Expression levels of Fas antigen and FasL were decreased in ATRA treatment group，compared with those in model group，P<0.05. Real-time PCR analysis showed that mRNA expression levels of Fas antigen and FasL in model group were （0.601 3±0.249 9） and （0.729 9±0.730 4），significant－ly high than those in ATRA treatment group（both P<0.05）. Conclusion：ATRA can protect kidney damage through decreasing the expression of Fas and FasL.