Effect of low frequency pulsed electromagnetic field on the expression and secretion of IL-1β and TNF-α in rabbit’s knee chondrocytes

Abstract:

Objective：To investigate the changes of the expression and secretion of interleukin-1β（IL-1β） and tumor necrosis factor-α（TNF-α） in rabbit’s knee chondrocytes under the stimulation of low frequency pulsed electromagnetic field（LEPEMF）. Methods：Marrow stem cells（MSCs） of rabbits were obtained and differentiated into chondrocytes in vitro. PEMF with different intensities was applied to stimulate the chondrocytes. The expression levels of IL-1β and TNF-α in the chondrocytes were measured by Western blot and their levels in the cultured medium were measured by enzyme linked immunosorbent assay（ELISA）. Result：PEMF did not exert obvious effect on the expression levels of IL-1β and TNF-α in the chondrocytes but it can reduce the secretion levels of of IL-1β and TNF-α in the cultured medium in a dose-dependent way. Conclusion：PEMF can inhibit the secretion of IL-1β and TNF-α in cultured rabbit’s chondrocytes.