Expression and function of CUG-binding protein 1 gene in prostate carcinoma

Abstract:

Objective：To study on the expression and function of CUG-binding protein 1（CUGBP1） in prostate carcinoma. Methods: The CUGBP1mRNA levels in the prostate carcinoma specimen and the adjacent non-carcinoma tissues were detected by quantitative RT-PCR. The expressions of four kinds prostate carcinoma cell strains（22RV1，PC-3，DU145，LNCaP clone FGC） in CUGBP1 were detected by Western blot. The proliferation and apoptosis of cell line PC-3 after silencing CUGBP1 were examed by RNA interference technique. The mRNA levels of downstream related genes in cell line PC-3 were determined by quantitative RT-PCR. Results：CUGBP1 was highly expressed in PC-3 and 22RV1. The CUGBP1 mRNA level in prostate carcinoma tissues was higher than that in adjacent non-carcinoma tissues. The CUGBP1 gene silencing，which was induced by shRNA lentivirus，suppressed the proliferation and apoptosis of cell line PC-3 and increased the proportion of cells in G1 phase. The mRNA level of CDKN1A in PC-3 cell line was up-regulated while that of BCL2，PCNA，MCM2 and MCM3 was down-regulated after CUGBP1 gene silencing. Conclusion：CUGBP1 gene may regulate the apoptosis，proliferation and cell cycle of prostate carcinoma cells through up-regulating and down-regulating downstream genes.