Correlation between single nucleotide polymorphisms at three sites of Klotho gene with coronary heart disease

Abstract:

Objective：To investigate single nucleotide polymorphisms（SNPS） at three sites of the Klotho（KL） gene and to analyze their correlation with coronary heart disease（CHD）. Methods：Totally 317 patients were randomly selected as CHD group and 301 healthy people as normal controls. Genomic DNA of leukocytes was extracted from venous blood and the single allele specific primer PCR technique（SASP-PCR） was used to detect allelic genotypes at three SNPS sites of KL gene in order to analyze their relationship with CHD. Results：The frequency of allele AA genotype of KL G-395A in CHD group was obviously higher than that in normal con－trol group（P<0.05）. According to the results of sex stratification analysis，the frequency of allele AA genotype in the CHD male sub－group was higher than that in normal control group（P<0.05）. The frequency of allele CC genotype of KL C370S was higher than that in normal control group（P<0.05），but the frequency of allele CS genotype was lower than that in normal control group（P<0.05）. Accord－ing to the results of sex stratification analysis，the frequency of allele CC genotype in CHD male subgroup was higher than that in normal control group（P<0.05），but the frequency of allele CS genotype was lower than that in normal control male subgroup（P<0.05）. There was no significant difference in polymorphic FF，FV and VV frequency in the F-352V gene between CHD group and normal control group（P >0.05）. According to the results of combination genotype analysis，the allele genotype frequency of KL G-395A+F352V+C370C，KL A-395A+F352V+C370C and KL A-395A+F352V+C370S in CHD group was higher than that in normal control group（P<0.05），but the allele genotype frequency of KL G-395A+F352V+C370S was lower than that in normal control group（P<0.05）. According to the results of sex stratification analysis，the allele genotype frequency of KL G-395A+F352V+C370C，KL A395-A+F352V+C370C was higher than that in normal control male subgroup（P<0.05），but the allele genotype frequency of KL G-395A+F352V+C370S was lower than that in normal control male subgroup（P<0.05）. The allele genotype frequency of KL A-395A+F352V+C370S in CHD female subgroup was obviously higher than that in normal control female subgroup（P<0.05）. Conclu－sions：This study shows that the male patients who carried with allele genotype KLG-395A SNP AA have higher risk in suffering from CHD. The male patients with allele CS genotype of KL C370S have lower risk in suffering from CHD. This study also finds that the male patients who carried with KL G395-A+F352V+C370C，KL A-395A+F352V+C370C and the female patients who carried with KL A-395A+F352V+C370S have higher risk in suffering from CHD，however，the male patients with KL G-395A+F352V+C370S have lower risk in suffering from CHD.