Effects of Ketamine on lipopolysaccharide-induced nuclear factor-kappaB expression of human umbilical vein endothelial cells

Abstract:

Objective：To investigate the effects of Ketamine on lipopolysaccharide（LPS）-induced translocation of nuclear factor-kappaB（NF-κB） in human umbilical vein endothelial cell nuclei and to explore it’s anti-inflammatory action. Methods：Human um－bilical vein endothelial cells（HUVECs） were divided into four groups:group C（blank control group），group L（1 μg/ml LPS was added），group KL1（3 μg/ml ketamine and 1 μg/ml LPS were added） and group KL2（30 μg/ml ketamine and 1 μg/ml LPS were added）. The tanslocation and expressions of NF-κB p65 in endothelial cell nuclei were detected by Western blot，reverse transcription polymerase chain reaction（RT-PCR） and image analysis system of HPIAS 2000. Results：The tanslocation and expression of NF-κB p65 in en－dothelial cell nuclei induced by LPS were reduced in group KL1 and KL2. Conclusions：The tanslocation and expression of NF-κB p65 in endothelial cell nuclei can be induced by LPS and Ketamine can suppress the activation.There are positive correlations be－tween Ketamine concentration and its inhibitory effect. The anti-inflammatory effect of Ketamine may be achieved by the translocation of ?资B.